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Furthermore, when compared to monotherapy with oxaliplatin and sorafenib, hCEC-Exo-214 in mixture with both drug considerably lessened protein degrees of P-glycoprotein (P-gp) and splicing factor 3B subunit 3 (SF3B3) in HCC cells. In conclusion, the present in vitro research provides evidence that hCEC-Exo-214 substantially improves the anti-tumor efficacy of oxaliplatin and sorafenib on HCC cells. HCC cells to sorafenib and doxorubicin, respectively. Quantitative RT-PCR investigation showed that, in contrast to regular human liver epithelial cells (THLE-2), HepG2 and Hep3B cells exhibited an approximate fifty and 70% significant reduction in miR-214 expression, respectively, whilst miR-92 expression was upregulated in HCC cells (Figure 1A). Additionally, we calculated miR-214 concentrations in liver tumor tissues acquired from sufferers with HCC who had gone through tumor resection. We examined miR-214 expression in two HCC cell traces, HepG2 and Hep3B. NTA analysis reveals particle distributions of EVs isolated from lifestyle medium of hCEC cells transfected by miR-214. R-214 transfected (hCEC-Exo-214) hCEC cells.